犬细小病毒高效纳米PCR检测方法的建立及初步应用

doi:10.11843/j.issn.0366-6964.2019.06.017

Abstract

Abstract: In order to establish a rapid and sensitive nanoparticle-assisted PCR (nano PCR) assay for detection of canine parvovirus (CPV), a pair of primers was designed based on the conserved region of the VP2 gene to amplify 574 bp fragment. The annealing temperature, primer concentration and other reaction conditions of nano PCR were optimized, and its specificity and sensitivity were evaluated. The results showed that the established nano PCR had good specificity and sensitivity, with a minimum nucleic acid detection amount of 2 copies·μL^-1, and its sensitivity was 1 000 times higher than that of conventional PCR. Seventy-five clinical samples collected from Beijing, Guangxi and Jilin were tested, the positive rate of CPV was 89.3% (67/75) and 70.7% (53/75) by nano PCR and conventional PCR, respectively. The results showed that the method had higher sensitivity and was suitable for the detection of low amount of CPV in clinical samples. The established nano PCR in this study provided a new method for rapid, sensitive and accurate diagnosis of CPV infection in the early stage,which laid a foundation for the prevention and control of CPV and had significant value in clinical application.

CLC Number:

S852.659.2

QIN Tong, ZHOU Ling, YOU Xinyue, LIANG Lin, SHI Lijun, ZHANG Jianwei, LI Jinxiang, CUI Shangjin. Establishment and Application of Nano PCR Assay for Detection of the Canine Parvovirus[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(6): 1268-1274.

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Establishment and Application of Nano PCR Assay for Detection of the Canine Parvovirus

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